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rhabdomyosarcoma (rd) cells jcrb9072  (JCRB Cell Bank)

 
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    JCRB Cell Bank rhabdomyosarcoma (rd) cells jcrb9072
    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected <t>RD</t> <t>cells</t> incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).
    Rhabdomyosarcoma (Rd) Cells Jcrb9072, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rd+cells+jcrb9072/rd+cells+jcrb9072/pmc11206571-182-0-8
    Average 90 stars, based on 1 article reviews
    rhabdomyosarcoma (rd) cells jcrb9072 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Novel Anti-Enterovirus A71 Compounds Discovered by Repositioning Antivirals from the Open-Source MMV Pandemic Response Box"

    Article Title: Novel Anti-Enterovirus A71 Compounds Discovered by Repositioning Antivirals from the Open-Source MMV Pandemic Response Box

    Journal: Pharmaceuticals

    doi: 10.3390/ph17060785

    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected RD cells incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).
    Figure Legend Snippet: Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected RD cells incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).

    Techniques Used: Activity Assay, Infection, Virus, Incubation, Control, Cell Culture, Sulforhodamine B Assay, Derivative Assay, Whisker Assay

    Related Articles

    other:

    Article Title: Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells.
    Article Snippet: Three ERMS cell line stocks were provided by JCRB Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan): ERMS1 cells (JCRB1648) derived from the anaplastic pelvic ERMS of a 5-year-old female [5], KYM1 cells (JCRB0627) derived from the neck ERMS of a 9-month-old infant [28], and RD cells (JCRB9072) derived from the malignant pelvic ERMS of a 7-year-old female [29].

    Article Title: Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells
    Article Snippet: Three ERMS cell line stocks were provided by JCRB Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan): ERMS1 cells (JCRB1648) derived from the anaplastic pelvic ERMS of a 5-year-old female [ ], KYM1 cells (JCRB0627) derived from the neck ERMS of a 9-month-old infant [ ], and RD cells (JCRB9072) derived from the malignant pelvic ERMS of a 7-year-old female [ ].



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    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected <t>RD</t> <t>cells</t> incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).
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    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected <t>RD</t> <t>cells</t> incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).
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    human rhabdomyosarcoma (rd) cells jcrb9072  (JCRB Cell Bank)
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    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected <t>RD</t> <t>cells</t> incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).
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    Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected RD cells incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).

    Journal: Pharmaceuticals

    Article Title: Novel Anti-Enterovirus A71 Compounds Discovered by Repositioning Antivirals from the Open-Source MMV Pandemic Response Box

    doi: 10.3390/ph17060785

    Figure Lengend Snippet: Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected RD cells incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).

    Article Snippet: Rhabdomyosarcoma (RD) cells (JCRB9072) were purchased from the Japanese Collection of Research Bioresources Cell Bank and grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, New York, NY, USA) supplemented with 1× GlutaMAX (Gibco, New York, NY, USA), 10% heat-inactivated fetal bovine serum (HyClone, Waltham, MA, USA), L-glutamine, and penicillin/streptomycin antibiotics (Gibco, New York, NY, USA), namely completed DMEM.

    Techniques: Activity Assay, Infection, Virus, Incubation, Control, Cell Culture, Sulforhodamine B Assay, Derivative Assay, Whisker Assay